Reduced killing of N. We wound inoculated potato tuber slices and chicory leaves and measured the resulting weight of macerated tissue potato and size of necrotic lesion chicory leaf but could detect no phenotype for the mutant. Documents which have cited the same bibliography. Full description at Econpapers Download paper. FHA is proteolytically processed after secretion, has multiple substrate-binding domains and proapoptotic activity, and promotes bacterial aggregation Closer inspection of the pathogenic defect in hecA mutant CUCPB revealed three events, attachment, aggregation, and epidermal cell killing, which occur in sequence before the appearance of disease symptoms and that appear to represent the earliest steps in E. 
| Uploader: | Tuk |
| Date Added: | 8 June 2006 |
| File Size: | 61.57 Mb |
| Operating Systems: | Windows NT/2000/XP/2003/2003/7/8/10 MacOS 10/X |
| Downloads: | 36793 |
| Price: | Free* [*Free Regsitration Required] |
Other factors that may contribute to virulence in different hosts include siderophores and exopolysaccharide systemic invasion of Saintpauliathe Sap system resistance to antimicrobial peptides in potatoand methionine sulfoxide reductase for repair of oxidative stress macerated lesions in pddf leaves 28 — Full description at Econpapers Download paper Benefits from delay?
riderpucel.tk
Received Jun Documents which have cited the same bibliography. An expanded version of this figure, including GenBank accession nos. Several plasmids expressing fluorescent proteins were tested in E.
Full description at Econpapers Download paper Effects of state contraceptive insurance mandates. Supplementary Material Supporting Information: Aggregates were observed occasionally in stomates, but they formed predominantly on the surface of the leaf, and 8 h after nbf there was no evidence that bacteria had invaded the mesophyll.
HecA and pef proteins found in other plant pathogenic bacteria are highly diverse. The importance of pectic enzymes in the active maceration phase of E. Discussion The soft-rot diseases caused by E.
Citation data for document RePEc:nbr:nberwo
Full description at Econpapers Download paper The effect of culture on the fertility decisions of immigrant women in the United States. The two-partner secretion domains of FHA-like adhesins found in plant pathogens are aligned, and conserved residues are highlighted.
The hecA gene was sequenced and mutagenized with Tn 7 in E. Full description at Econpapers Download paper High school dropout and teen childbearing. The GenBank accession no.
NBR Desenho tecnico - Dobramento de
To determine whether the relative similarities of these proteins correlate with the phylogeny of the bacteria, a test of congruence between the secretion domain from all plant and animal 1314 and a 16S rRNA data set was conducted. The attachment studies also revealed that 4 h after inoculation, bacteria began hbr form aggregates that eventually became large clumps of cells immobilized on the leaf surface. In contrast, the role of attachment and adhesins in virulence is unclear for the more prevalent necrogenic rather than tumorigenic plant pathogens.
A The time course of macroscopically observable necrosis in seedlings was determined after inoculation as described in Fig. Full description at Econpapers Download paper Singleton status and childhood obesity: The location of the transposon insertions was determined by sequencing outward of the transposon by using Tn 7 primers gpsS and gpsN, provided by the manufacturer. This work was supported by U. Cell killing also depended on pvf of the major pectate lyase isozymes and the type II, but not the ;df III, secretion pathway in E.
Here, we have found that HecA, a member of a class of adhesins important in several animal pathogens, is important in the virulence of E. Abbreviations Hec hemolysin-like protein in E.
Full description at Econpapers Download paper The impact of abortion legalisation on birth outcomes in Uruguay. Radulescu, Doina ; Egger, Peter.
The large size of these proteins, the presence of multiple copies in some genomes, and the potential redundancy for submissions of partial protein sequences led us to restrict the data set for the analysis to the well characterized B.
The overall structure of all of the FHA-like adhesins found by blastx search Table 2, which is published as supporting information on the PNAS web site is similar, so we assumed that nbt entire proteins are homologous in these taxa.
However, they share structural features of FHA-like adhesins, including large size, the presence of an N-terminal signal peptide, a aa N-proximal two-partner secretion domain, Gly-rich regions, repetitive sequences, and multiple Arg-Gly-Asp motifs. Closer inspection of the pathogenic defect in hecA mutant CUCPB revealed three events, attachment, aggregation, and epidermal cell killing, which occur in sequence before nb appearance of disease symptoms and that pdff to represent the earliest steps in E.
The entire hecA sequence was used to search the GenBank nonredundant database by using the blastx program Foundation N B R, editor. HecA homologs were found in bacterial pathogens of plants and animals and appear to be unique to pathogens and universal in necrogenic plant pathogens. These observations indicate that this early killing of pdff cells depends on HecA and pectic enzymes but is independent of the Hrp system.
No comments:
Post a Comment